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Parallel and antiparallel triple helices with G,A-containing third strands

✍ Scribed by Horea Porumb; Hervé Gousset; Eliane Taillandier

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
87 KB
Volume
20
Category
Article
ISSN
0173-0835

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✦ Synopsis

A triple helix, formed by a 13 nucleotide (nt) all-purine oligonucleotide, containing six contiguous guanines, oriented parallel to a homopurine strand present in the polypurine tract of Friend leukemia virus, was obtained in 0.1 M LiCl. Its dissociation constant at 25 o C, given by electrophoretic titration, of the order of 50 nM, is at least ten times lower than that of the corresponding antiparallel triplex formed on the same target. At 4 o C, the parallel orientation of the homopurine strands is favored to the point that the guanine block of 6 nt, present in the antiparallel' oligonucleotide, attaches in a parallel fashion to the corresponding block in the target strand, to generate a partial, parallel triplex, that coexists with the antiparallel one.

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