Paper electrophoresis of nicotinic acid derivatives

✍ Scribed by T.K. Sundaram; K.V. Rajagopalan; P.S. Sarma

• Publisher: Elsevier Science
• Year: 1959
• Tongue: English
• Weight: 413 KB
• Volume: 2
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)86337-8

✦ Synopsis

Studies on the metabolism of nicotinic acid and nicotinamide have revealed that the deamidation of nicotinamide constitutes an important aspect of the vitamin metabolism in several organisms and indeed appears to be the major metabolic pathway in the mould Neurospora crassat and the insect, Corcyra cephalonica St .2. The tissues of the pigeon and the chick have also been found to have particularly strong nicotinamide deamidase activity3 . These earlier studies have involved a preliminary qualitative detection of deamidase activity through the application of the paper chromatographic technique and the subsequent quantitative determination of enzyme activity by a microbiological assay procedure using the organism, Leuconostoc mesenteroides 9x354 .