Paper chromatography of the most polar α-ketolic steroids

✍ Scribed by Joseph C. Touchstone; Milton R. Horwitz

• Publisher: Elsevier Science
• Year: 1963
• Tongue: English
• Weight: 188 KB
• Volume: 6
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(63)90155-6

✦ Synopsis

Studies on isolation of cr-ketolic steroids more polar than tetrahydrocortisol, particularly 6P-hydroxycortisol, have been hampered by lack of suitable solvent systems for their separation by paper chromatography. Using water solubility as criterion we selected methylene chloride and fluorobenzene, both more polar than toluene, as the mobile phases of the two new systems in which ethylene glycol served as stationary phase. Comparisons of these two new systems with the toluene-ethylene glycol (T-EG) system were made. The chloroform-formamide and butyl acetate-formamide (2) systems have been used for this purpose in this laboratory

(3) but, although rapid, they have the disadvantage that certain supplies of either chloroform or formamide have not been satisfactory due to instability.