Paper-based microfluidic devices for analysis of clinically relevant analytes present in urine and saliva

✍ Scribed by Scott A. Klasner; Alexander K. Price; Kurt W. Hoeman; Rashaun S. Wilson; Kayla J. Bell; Christopher T. Culbertson

• Publisher: Springer
• Year: 2010
• Tongue: English
• Weight: 334 KB
• Volume: 397
• Category: Article
• ISSN: 1618-2650
• DOI: 10.1007/s00216-010-3718-4

✦ Synopsis

We report the use of paper-based microfluidic devices fabricated from a novel polymer blend for the monitoring of urinary ketones, glucose, and salivary nitrite. Paper-based devices were fabricated via photolithography in less than 3 min and were immediately ready for use for these diagnostically relevant assays. Patterned channels on filter paper as small as 90 μm wide with barriers as narrow as 250 μm could be reliably patterned to permit and block fluid wicking, respectively. Colorimetric assays for ketones and nitrite were adapted from the dipstick format to this paper microfluidic chip for the quantification of acetoacetate in artificial urine, as well as nitrite in artificial saliva. Glucose assays were based on those previously demonstrated (Martinez et al.,