Pancreatic cancer in europe: Ki-ras gene mutation pattern shows geographical differences
✍ Scribed by Aldo Scakpa; Paola Capelli; Alberto Villanueva; Giuseppe Zamboni; Felix Lluì; Roberto Accolla; Gianmario Mariuzzi; Gabriel Capellà
- Book ID
- 102865630
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 919 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Seventy-seven pancreatic adenocarcinomas (60 Spanish and 17 Italian) were tested for Ki-ras gene mutations by analysis of polymerase chain reaction amplified sequences. Mutations involving codon I 2 GGT; gly) were detected in I6 Italian and 46 second base and were G to A transitions GAT; asp) in 8 cases Forty-two Spanish mutations w e 6 characterized. Thirty-eight were at the second and 4 at the fiirst base: asp in 24 cancers, val in 14, arg (CGT) in 2 and cys @G in 2. Previous European atic cancers harbored a codon I 2 Ki-ras mutation (8O%), the large majority affecting the second base (73Yo). with a transitions/transversions ratio of I .3: I. However, the mutational pattern of cancers of the different European countries shows remarkable differences, both in the site of the mutation (first or second base) and in the ratio of t:ransitions over transversions. Moreover, a significant subgroup of pancreatic carcinomas do not harbor Ki-ras mutations. The classification of pancreatic cancers, according to the preseince or absence, and type of Ki-ras mutation, may be of imiportance in epidemiological studies. A critical reappraisal of existing epidemiological data, through a retrospective genotypic study using paraffin-embedded cancer samples, may reveal significant correlations with specific genotoxic agents.