PAMAM Dendrimers as Potential Agents against Fibrillation of α-Synuclein, a Parkinson's Disease-Related Protein
✍ Scribed by Agata Rekas; Victor Lo; Gerry E. Gadd; Roberto Cappai; Seok I. Yun
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 644 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1616-5187
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✦ Synopsis
Abstract
The effect of PAMAM dendrimers (generations G3, G4 and G5) on the fibrillation of α‐synuclein was examined by fluorescence and CD spectroscopy, TEM and SANS. PAMAM dendrimers inhibited fibrillation of α‐synuclein and this effect increased both with generation number and PAMAM concentration. SANS showed structural changes in the formed aggregates of α‐synuclein – from cylindrical to dense three‐dimensional ones – as the PAMAM concentration increased, on account of the inhibitory effect. PAMAM also effectively promoted the breaking down of pre‐existing fibrils of α‐synuclein. In both processes – that is, inhibition and disassociation of fibrils – PAMAM redirected α‐synuclein to an amorphous aggregation pathway.
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