Palmitoylated proteins: purification and identification
โ Scribed by Wan, Junmei; Roth, Amy F; Bailey, Aaron O; Davis, Nicholas G
- Book ID
- 109946210
- Publisher
- Nature Publishing Group
- Year
- 2007
- Tongue
- English
- Weight
- 313 KB
- Volume
- 2
- Category
- Article
- ISSN
- 1750-2799
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## Abstract Infantile neuronal ceroid lipofuscinosis (INCL; Batten disease) is a severe neurodegenerative disorder of childhood characterized by the accumulation of autofluorescent storage material in lysosomes. It is caused by mutation of the __CLN1/PPT1__ gene, which encodes the lysosomal enzyme
Maize root membranes were extracted and the solubilized proteins were affinity-purified using an ABA-BSA-Sepharose 4B matrix. The retained proteins were eluted with 4 M urea or M ABA. ABA could elute the binding proteins but other phytohormones, such as M A or GAS, could not. ABA binding activity wa