Paired H-2-specific monoclonal antibodies react differently to red cells and lymphocytes

✍ Scribed by Susan L. Tonkonogy; D. Bernard Amos

• Book ID: 104734589
• Publisher: Springer-Verlag
• Year: 1981
• Tongue: English
• Weight: 472 KB
• Volume: 14
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00350121

✦ Synopsis

Two hybrid clones from a fusion of C57BL/6 anti-DBA/2 spleen cells and the myeloma line Sp2/0 secrete antibodies reactive with a product of the murine major histocompatibility complex (MHC). The two antibodies are provisionally designated S 13.1 l and S 13.29. Both react in rabbit-complementmediated cytotoxicity with spleen cells of H-2 d, H-2 ~, H-2 r, and H-2 p strains. In addition, both antibodies hemagglutinate red blood cells from these strains. S13.11 is also cytotoxic for H-2 a, H-2 k, H-2", and H-2 v spleen cells but does not hemagglutinate red blood cells from mice bearing these haplotypes. With the exception of H-2 ~, this strain pattern mimics the public specificity H-2.8. Quantitative absorption of S13.11 shows that H-2 ~ cells are twice as efficient as H-2 k cells in their ability to remove the S 13.11 antibody. S 13.29 reacts weakly in cytotoxicity with H-2 k spleen cells and does not react with cells from H-2 u or H-2 ~. Blocking studies indicate that S13.11 and S13.29 react with the same or a closely related molecule on the cell surface.