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Packing procedure of silica columns for HPLC with aqueous slurries

✍ Scribed by Claessens, H. A. ;Aben, G. ;Vonk, N.

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 295 KB
Volume: 5
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240050505

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✦ Synopsis

Abstract

Successful packing of high quality HPLC columns is dependent upon a number of parameters. Especially when using slurry packing techniques, the absence of conglomerates of particles in the slurry and the wettability of the particles are important. The method described in this paper fulfills these conditions, imparting a ξ potential to the 5 and 10 μm silica particles by applying a pH gradient. This results in repulsive forces between the particles and hence leads to a well‐controlled monodisperse situation. Water is used as a slurry and displacement liquid; it combines the advantages of excellent wetting properties and non‐toxicity.

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