Packed column SFC/UV versus HPLC/UV analysis of valerenic acids and valepotriates in extracts of Valeriana officinalis L.
✍ Scribed by Carlo Bicchi; Arianna Binello; Patrizia Rubiolo
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 69 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Valerenic acids and valepotriates are considered among the constituents responsible for the sedative effect of roots and rhizomes of Valeriana species. Packed column SFC/UV (p-SFC) was applied to the analysis of valerenic acids and valepotriates extracted from the underground parts of Valeriana officinalis. The separation of these compounds by p-SFC was optimized by varying the conditions (i.e. columns, column coupling and stationary phase coupling, density programmes, types of modifiers and modifier programmes). A CN-stationary phase using supercritical carbon dioxide modified with methanol:water (95:5) as mobile phase was successful in separating both valerenic acids and valepotriates. The qualitative and quantitative results of p-SFC are comparable to HPLC for both valerenic acids and valepotriates, but analyses are faster with p-SFC than with HPLC.