Packed capillary HPLC: An attractive separation technique for small organic molecules
✍ Scribed by Bruns, Andreas ;Polta, John
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 328 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
The feasibility of using packed capillary HPLC for the analysis of small organic molecules has been demonstrated by three examples: the separation of glucose and sucrose telomers, the separation of the different components of a solvent extraction reagent, and the separation of mono‐, di‐, and trioleins, all of which have been achieved with packed capillary columns of 0.32 mm inner diameter and eluent flow rates of 3 microliters per minute. Application to the analysis of the solvent extraction reagents has shown that this technique can be used as a quantitative tool in just the same way as any conventional HPLC method.
The feasibility of a direct packed capillary HPLC – mass spectrometry interface has been demonstrated for the glucose telomer separation; direct coupling is only possible because of the microliter flow rates employed by the technique.