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Packed-bed reactor of immobilized bacterial bioluminescence enzymes: A potential high-sensitivity detector for automated analyzers

✍ Scribed by Kalevi Kurkijärvi; Raimo Raunio; Timo Korpela


Publisher
Elsevier Science
Year
1982
Tongue
English
Weight
402 KB
Volume
125
Category
Article
ISSN
0003-2697

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✦ Synopsis


Commercial bacterial bioluminescence enzymes were immobilized on agarose with 85% recovery of coupled activity. A light-emitting continuous-flow column for NADH monitoring at picomole levels was developed. The present enzyme reactor exhibited good operational stability in continuous use, which makes the system applicable to a sensitive detector for automated analyzers.