PAC clone containing the HMGI(Y) gene spans the breakpoint of a 6p21 translocation in a uterine leiomyoma cell line
✍ Scribed by B. Kazmierczak; S. Bol; S. Wanschura; S. Bartnitzke; J. Bullerdiek
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 243 KB
- Volume
- 17
- Category
- Article
- ISSN
- 1045-2257
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✦ Synopsis
Akin to the HMGI-C rearrangements observed in benign solid tumors with 12q 14-I 5 abnormalities, the HMG/(Y) gene has been assumed to play a crucial role in tumors with 6p21 abnormalities. Fluorescence in situ hybridization (FISH) studies using a PAC clone containing the HMGI(Y) gene as a molecular probe have been performed on a cell line from a uterine leiomyoma with a complex translocation involving chromosomal band 6p2 I .3. The results revealed that the breakpoint mapped within the PAC clone as reflected by signals on the normal chromosome 6 and both derivative chromosomes I and 14. Thus, the breakpoint was located within the HMGIC/) gene or its close vicinity. These findings support the idea that HMGI(Y) rearrangements are causally related to the origin of uterine leiomyomas with 6p2 I abnormalities.