PAA-Derived Gold Nanorods for Cellular Targeting and Photothermal Therapy

✍ Scribed by Dickson K. Kirui; Sushmitha Krishnan; Aaron D. Strickland; Carl A. Batt

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 1012 KB
• Volume: 11
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.201100050

✦ Synopsis

Abstract

A single‐step LbL procedure to functionalize CTAB‐capped GNRs via electrostatic self‐assembly is reported. This approach allows for consistent biomolecule/GNR coupling using standard carboxyl‐amine conjugation chemistry. The focus is on cancer‐targeting biomolecule/GNR conjugates and selective photothermal destruction of cancer cells by GNR‐mediated hyperthermia and NIR light. GNRs were conjugated to a single‐chain antibody selective for colorectal carcinoma cells and used as probes to demonstrate photothermal therapy. Selective targeting and GNR uptake in antigen‐expressing SW 1222 cells were observed using fluorescence microscopy. Selective photothermal therapy is demonstrated using SW 1222 cells, where >62% cell death was observed after cells are treated with targeted A33scFv‐GNRs. magnified image