p53 Protein alterations in human testicular cancer including pre-invasive intratubular germ-cell neoplasia
✍ Scribed by Jir̂ina Bártkov´; Jir̂í Bártek; Jir̂ina Lukáŝ; Bor̂ivoj Vojtêŝek; Zdenka Staŝková; Aleŝ Rejthar; Jan Kovar̂ík; Carol A. Midgley; David P. Lane
- Publisher
- John Wiley and Sons
- Year
- 1991
- Tongue
- French
- Weight
- 942 KB
- Volume
- 49
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Expression of the p53 oncoprotein was examined in a wide range of primary human testicular germ-cell tumours using a new mouse monoclonal antibody (MAb) BP53-I I raised and characterized in this study, in parallel with a polyclonal rabbit antiserum CM-I. lmmunohistochemistry on paraffin sections showed positive nuclear reaction in at least a fraction of malignant cells in 90 (84%) out of 107 cases studied. Aberrant accumulation of the p53 protein was found among testicular tumours of all major histological types, although generally a higher percentage of positive cases and a higher proportion of p53 over-expressing nuclei within individual lesions was observed in embryonal carcinomas when compared with seminomas. The typical heterogeneous staining pattern characteristic of histological specimens was also found in a cultured cell line derived from a human embryonal carcinoma. In contrast to immunohistochemically undetectable levels in normal testes and morphologically normal tissue areas in the tumourbearing testes, the accumulation of the p53 protein was clearly identified in a high proportion (59% of cases) of the pre-invasive lesions with positive atypical intratubular germ cells often found in the tissue adjacent to invasive tumours. Altered expression of the p53 protein is therefore a unifying feature of the majority of invasive male germ-cell tumours and the change resulting in high levels of p53 appears to be a relatively early step in the human testicular cancer pathogenesis.