p53 gene mutations in neoplastic transformation of C3H 10T1/2 and severe combined immunodeficiency fibroblasts
✍ Scribed by S Lang; M Marchesani; K Servomaa; L Kauppinen; J Jänne; T Rytömaa; R Wells; M Lun; M.M Elkind
- Book ID
- 117614655
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 65 KB
- Volume
- 434
- Category
- Article
- ISSN
- 0921-8777
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## Abstract The denaturing‐gradient gel electrophoresis (DGGE) blot technique is a highly sensitive method developed to detect sequence differences (e.g., single base changes and small deletions) in genomic DNA. We used this method to screen for mutations within the __p53__ locus of independent X‐r
We have examined the pattern of protein myristoylation in C3H10T1/2 fibroblasts during cell growth. During the growing phase of 10T1/2 cells, several proteins were radiolabelled with [3H]myristate, and among them proteins with molecular masses of 22, 35, a doublet of 42-45 and 67 kDa were labelled p