P393 PLATELET-DERIVED GROWTH FACTORS ENHANCE PROLIFERATION AND DIFFERENTIATION OF HUMAN ARTICULAR CHONDROCYTES
β Scribed by A. Spreafico; F. Chellini; S. Niccolini; D. Benetti; T. Serchi; A. Santucci; B. Frediani; R. Marcolongo
- Book ID
- 114349732
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 42 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1063-4584
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Dedifferentiated human articular chondrocytes exhibited a wide variation in their capacity to proliferate and redifferentiate in an alginate suspension culture system. The greatest extent of proliferation and redifferentiation was seen to be dependent on the formation of clonal populations of chondr
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Adult human articular chondrocytes were expanded in a medium with 10% serum (CTR) or further supplemented with different mitogens (i.e., EGF, PDGFbb, FGF-2, TGFb1, or FGF-2/TGFb1). Cells were then induced to redifferentiate in 3D pellets using serum-supplemented medium (SSM), serum-free medium (SFM)