P2Y2 receptors induced cell surface redistribution of αv integrin is required for activation of ERK 1/2 in U937 cells

✍ Scribed by Nataliya E. Chorna; Migdalia Chevres; Cynthia Santos-Berrios; Elsie A. Orellano; Laurie Erb; Fernando A. González

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 605 KB
• Volume: 211
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.20946

✦ Synopsis

Abstract

Nucleotides released from cells due to stress, injury or inflammation, induce mitogenic effects in monocytes via activation of P2Y~2~ nucleotide receptors (P2Y~2~Rs). Here we show that P2Y~2~ nucleotide receptors in U937 monocytic cells regulate the activation of extracellular signal‐regulated kinases 1 and 2 (ERK 1/2) by inducing the clustering of α~v~ integrins. The activation of phosphatidylinositol 3‐kinase by P2Y~2~R ligands was required for α~v~ clustering, suggesting a means whereby two different classes of receptors communicate to induce mitogenic responses in monocytic cells. P2Y~2~R‐induced α~v~ clustering was also associated with a flattened phenotype of the U937 cells, consistent with the role of the P2Y~2~R in regulating early events in cell migration. J. Cell. Physiol. 211: 410–422, 2007. © 2006 Wiley‐Liss, Inc.