P040 Coagulation disorders and inhibitors of coagulation in children: cases from Mansoura, Egypt

✍ Scribed by N. Abdelrazik; H. Rashad; T. Selim; L. Tharwat

• Book ID: 104323105
• Publisher: Elsevier Science
• Year: 2007
• Tongue: English
• Weight: 48 KB
• Volume: 21
• Category: Article
• ISSN: 0268-960X
• DOI: 10.1016/s0268-960x(07)70115-7

✦ Synopsis

Materials and Methods: As lymphoblastoid cell lines (LCLs) are well established in vitro models for PTLD, we have assessed 17 LCLs for cytotoxic drug sensitivity. After three days of incubation, live and dead cells were differentially stained using fluorescent dyes. The precise numbers of live and dead cells were determined using a custom designed automated laser confocal fluorescent microscope. Results: Independently of their origin, LCLs showed very similar drug sensitivity patterns against 29 frequently used cytostatic drugs. LCLs were highly sensitive for vincristine, methotrexate, epirubicin and paclitaxel. Conclusion: Our data shows that the inclusion of epirubicin and paclitaxel into chemotherapy protocols against PTLD may be justified.