P-31 in-vivo NMR investigation on the function of polyphosphates as phosphate-and energysource during the regreening of the green algaChlorella fusca

✍ Scribed by A. C. Kuesel; J. Sianoudis; D. Leibfritz; L. H. Grimme; A. Mayer

• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 622 KB
• Volume: 152
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00456096

✦ Synopsis

The green alga Chloretla fusca accumulates polyphosphates under conditions of nitrogen starvation while deassembling the photosynthetic apparatus. The polyphosphate content of cells regreening after resupply with nitrate under different culture conditions was investigated by P-31 in-vivo NMR spectroscopy. Neither phosphate deficiency nor anaerobiosis during the first hours of regreening inhibited the recovery of the cells. Polyphosphates were degraded during regeening. Differences in the amount of polyphosphates of phosphate supplied and deficient cells occurred only after more then 8 h. After 16 h phosphate deficient cells had still 75 % of the polyphosphate content of phosphate supplied cells. In cells kept under anaerobic conditions polyphosphate degradation was much higher than in oxygen supplied cells. After 8 h they contained less than 50% of the polyphosphate content of oxygen supplied cells. These data suggest that polyphosphates serve as obligatory phosphate source during regreening and may be used as an energy source.