𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Oxygen gradients in CHO cells: Measurement and characterization by electron spin resonance

✍ Scribed by James F. Glockner; Harold M. Swartz; Mark A. Pals

Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
725 KB
Volume
140
Category
Article
ISSN
0021-9541

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✦ Synopsis

The concentration of oxygen within cells is important in many physiological and pathological processes, but such oxygen-dependent processes are generally studied as a function of the concentration of extracellular oxygen, due to a lack of suitable methods. Using a newly developed technique based on ESR spectroscopy, we show that respiration stimulation of a cell suspension can result in a significant difference between average intracellular and extracellular concentrations of oxygen. These results indicate that studies of oxygen-dependent phenomena in cells may require measurement of intracellular oxygen concentrations and imply that there are mechanisms in cells that restrict the free diffusion of oxygen.

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