Oxygen content of the fixative is import
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Maxwell, Linda ;Gavin, John Bevan ;Walker, Sharon
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Article
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1991
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Wiley (John Wiley & Sons)
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English
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Isolated rat hearts were subjected to 15,45, or 60 minutes of global ischaemia and then fixed by perfusion at 37ยฐC with glutaraldehyde containing various amounts of oxygen. This either had been bubbled with 100% oxygen (PO, 620 mm Hg) or with 100% nitrogen (PO, 40 mm Hg) immediately before use, or i