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Oxygen-dependent delayed fluorescence measured in skin after topical application of 5-aminolevulinic acid

✍ Scribed by Floor A. Harms; Wadim M. I. de Boon; Gianmarco M. Balestra; Sander I. A. Bodmer; Tanja Johannes; Robert J. Stolker; Egbert G. Mik

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
986 KB
Volume
4
Category
Article
ISSN
1864-063X

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✦ Synopsis

Abstract

Mitochondrial oxygen tension can be measured in vivo by means of oxygen‐dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that delayed fluorescence is readily observed from skin in rat and man after topical application of the PpIX precursor 5‐aminolevulinic acid (ALA). Delayed fluorescence lifetimes respond to changes in inspired oxygen fraction and blood supply. The signals contain lifetime distributions and the fitting of rectangular distributions to the data appears more adequate than mono‐exponential fitting. The use of topically applied ALA for delayed fluorescence lifetime measurements might pave the way for clinical use of this technique. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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5-Aminolevulinic acid (ALA) is used as a precursor of protoporphyrin IX (PpIX) for photodynamic therapy (PDT) of superficial skin cancers and subcutaneous metastases of internal malignancies. The permeability of ALA across intact skin is always low, making it difficult to achieve the desired therape