Oxygen consumptions of interscapular brown adipose tissue homogenates from two species ofPeromyscus raised in different thermal environments
✍ Scribed by Mason, Elliott B. ;Prychodko, William
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 500 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
The oxygen uptakes of interscapular brown adipose tissue sucrose homogenates from two species of Peromyscus that were raised in the laboratory at environmental temperatures of 5 °C, 23 °C, and 33 °C were measured by direct Warburg manometry with β‐hydroxybutyrate as substrate. Determinations were carried out at 37 °C on homogenates made from the tissues of animals remaining at the temperatures at which they were raised as well as on homogenates made from the tissues of animals that had been exposed for ninety minutes to the other temperatures of the study.
Homogenates made from the interscapular brown adipose tissues of the northern species, P. maniculatus bairdii, raised at 23 °C and 33 °C consumed comparatively small amounts of oxygen, but tissue homogenate oxygen uptakes were significantly higher when P. maniculatus bairdii raised at 23 °C and 33 °C were exposed for short periods to 5 °C. Homogenates made from the interscapular brown adipose tissues of the southern species, P. polionotus, raised at 33 °C consumed small amounts of oxygen, but tissue homogenate oxygen uptakes were significantly higher following short term exposures of P. polionotus raised at 33 °C to both 5 °C and 23 °C.
The comparatively high oxygen uptakes of homogenates made from the interscapular brown adipose tissues of both species raised at 5 °C and P. polionotus raised at 23 °C did not decrease when animals raised under these conditions were exposed for short periods to warmer environments.
The results of the study indicate a cold adaptive role for interscapular brown adipose tissue that is consistent with the events of temperature acclimation in rodents as well as the fact that species specific differences in homogenate oxygen uptakes appear to be representative of genetically based environmental adaptations.