โ Scribed by Knobloch, Karl ;Ishaque, M. ;Aleem, M. I. H.
No coin nor oath required. For personal study only.
Cell-flee preparations from autotrophically grown M. denitri/icans yielded P/O ratios of 0.6--1.6 with H e, 0.4--1.2 with NADH, 0.7--1.0 with suceinate, and 0.3--0.5 with ascorbate as the oxidizable substrates.
The phosphorylation in all cases was inhibited effectively by DNP, DBP, PCP, CCCP, and dicumarol at concentrations which did not cause any significant inhibition of oxygen uptake.
The respiratory chain inhibitors such as antimycin A, HOQNO, cyanide, and azide were the potent inhibitors of the phosphate esterifieation coupled to the oxidation of H 2, NADtt, and suecinate; the ascorbate-linked phosphorylation was inhibited by cyanide or azide only.
While the NADH oxidation and associated phosphorylation was markedly sensitive to rotenone and other flavoprotein inhibitors, the oxidation of H 2 was relatively insensitive although there was a partial inhibition of the coupled phosphorylation. The experimental results indicated that bulk of the electron transfer from H~ bypassed the NADI-i-dehydrogenase or the rotenone sensitive site.
A series of articles have recently appeared concerning the electron transport and coupled energy metabolism in Micrococcus denitri]icans grown on suecinate (Asano et al., 1967a(Asano et al., , 1967b;;Imai et al., 1967 Imai et al., , 1968)).
These investigators concluded that the terminal site involving the cytochrome c:02 oxidoreduetase region of the electron transport chain was not involved in oxidative phosphorylation due probably to the lack of a * Non-standard Abbreviations : BAL = British Anti-Lewisite (2,3-Dimereaptopropanol),
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