Oxidative DNA damage from sulfite autoxidation catalyzed by manganese(III)

✍ Scribed by Rachel A. Jameton; James G. Muller; Cynthia J. Burrows

• Book ID: 104448221
• Publisher: Elsevier Science
• Year: 2002
• Tongue: English
• Weight: 103 KB
• Volume: 5
• Category: Article
• ISSN: 1631-0748
• DOI: 10.1016/s1631-0748(02)01405-4

✦ Synopsis

A series of manganese(II) and manganese(III) salts and oxides were investigated for their ability to catalyze the autoxidation of sulfite resulting in oxidative damage to DNA. Experiments directed at identifying the reactive intermediates responsible for DNA oxidation included the trapping of a sulfite radical adduct during EPR studies, alcohol quenching studies of free radical intermediates, comparisons of relative reactivities toward conversion of Type I to Type II plasmid DNA and cleavage of a duplex DNA restriction fragment. The studies support previous work on the mechanism of manganese(III)-catalyzed sulfite autoxidation and provide evidence that simple manganese salts are capable of mediating substantial DNA damage in conjunction with sulfite.