Oxidation of reduced pyridine nucleotides in Pasteurella tularensis: II. Separation of diaphorases and transhydrogenases
โ Scribed by Diana A. Robinson; R.C. Mills
- Book ID
- 115790080
- Publisher
- Elsevier Science
- Year
- 1961
- Weight
- 418 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0006-3002
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๐ SIMILAR VOLUMES
A simplified and reliable assay for the determination of erythrocyte pyridine nucleotide (NAD and NADP) concentrations, as well as the ratio of the reduced [NADH/NADPH] to oxidized \(\left[\mathbf{N A D}^{+} / \mathbf{N A D P}^{+}\right.\)] nucleotide, is important in understanding both normal and a
Several methods are available for the extraction and quantitation of oxidized and reduced pyridine nucleotides in erythrocytes. Enzymatic methods, however, are complicated by the presence of hemoglobin, which causes oxidation of NADH and NADPH during extraction. Although hemoglobin-mediated oxidatio