An extraction procedure using mixtures of phenol, chloroform, and isoamyl alcohol originally applied to quench mitochondria for determining adenylates proved suitable also for the quantification of reduced and oxidized pyridine nucleotides yielding recoveries of more than 90%. In combination with HP
Oxidation of Reduced Pyridine Nucleotides and of Sulphite by Pea Root Mitochondria
โ Scribed by STICKLAND, R. G.
- Book ID
- 109615534
- Publisher
- Nature Publishing Group
- Year
- 1961
- Tongue
- English
- Weight
- 222 KB
- Volume
- 190
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/190648a0
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๐ SIMILAR VOLUMES
A simplified and reliable assay for the determination of erythrocyte pyridine nucleotide (NAD and NADP) concentrations, as well as the ratio of the reduced [NADH/NADPH] to oxidized \(\left[\mathbf{N A D}^{+} / \mathbf{N A D P}^{+}\right.\)] nucleotide, is important in understanding both normal and a
Several methods are available for the extraction and quantitation of oxidized and reduced pyridine nucleotides in erythrocytes. Enzymatic methods, however, are complicated by the presence of hemoglobin, which causes oxidation of NADH and NADPH during extraction. Although hemoglobin-mediated oxidatio