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Oxidation of 6-hydroxydopamine catalyzed by tyrosinase

✍ Scribed by JoséNeptuno Rodriguez-López; Ramón Varón; Francisco García-Cánovas

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
713 KB
Volume
25
Category
Article
ISSN
0020-711X

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A procedure for calibrating a Clark-type oxygen electrode is described. This method is based on the oxidation of 4-tert-butylcatechol (TBC) by O2 catalyzed by tyrosinase, to yield 4-tert-butyl-o-benzoquinone (TBCQ). This reaction consumes known amounts of oxygen in accordance with the stoichiometry: