✦ LIBER ✦

Oxidase/peroxidase bilayer-modified electrodes as sensors for lactate, pyruvate, cholesterol and uric acid

✍ Scribed by Tetsu Tatsuma; Tadashi Watanabe

Book ID: 102623614
Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 393 KB
Volume: 242
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(91)87050-h

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✦ Synopsis

Enzyme heterobilayer-modified electrodes were fabricated by successively covalently binding to the surface of a tin(W) oxide plate horseradish peroxidase (HRP), then an oxidase (lactate, pyruvate or cholesterol oxidase or uricase), which liberates hydrogen peroxide by reaction with the respective substrate. The cooperative action of oxidase-HRP leads to an efficient amperometric sensor system with the minimum amount of enzyme immobilized on an electrode.

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