✦ LIBER ✦

Overproduction of recombinant proteins by escherichia coli — employing the combinations of two and three different types of multicopy plasmids — in a 60-litre working volume airlift tower loop reactor

✍ Scribed by Brandes, Lutz; Wu, Xiaoan; Maschke, Hans E.; Schügerl, Karl

Book ID: 123235913
Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 750 KB
Volume: 28
Category: Article
ISSN: 1873-3298
DOI: 10.1016/0032-9592(93)80003-Y

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Overproduction of EcoRI and SpA::EcoRI f

Overproduction of EcoRI and SpA::EcoRI fusion protein by Escherichia coli employing the combinations of three different types of multicopy plasmids: Comparison of plasmid stabilities and product concentrations

✍ Xiaoan Wu; Lutz Brandes; Hans E. Maschke; Karl Schügerl 📂 Article 📅 2007 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 381 KB

The copy numbers of the expression plasmids (pRTF309+ and pMTC48), the repression plasmids (pc1857 and pRK248cI) and the protection plasmid (pEcoR4) in recombinant E. coli JM 103 were investigated. In the absence of the protection plasmid, the copy number of the expression plasmid dropped; in its pr

Fed-batch cultivation of recombinant esc

Fed-batch cultivation of recombinant escherichia coli JM103 and production of the fusion protein SPA::EcoRI in a 60-L working volume airlift tower loop reactor

✍ Lutz Brandes; Xiaoan Wu; Jens Bode; Jong II Rhee; Karl Schügerl 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 852 KB

EcoRI fusion protein was produced by Escherichia coli JM103 carrying the multicopy expression plasmid pMTC48, the multicopy repressor plasmid pRK248, and the multicopy protection plasmid pEcoR4 in a 60-L working volume airlift tower loop reactor on M9 minimal medium with glucose. Cell mass concentra