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Overexpression, purification, crystallization and preliminary X-ray diffraction analysis of the pMV158-encoded plasmid transcriptional repressor protein CopG

✍ Scribed by F.Xavier Gomis-Rüth; Marı́a Solà; Rosa Pérez-Luque; Paloma Acebo; M.Teresa Alda; Ana González; Manuel Espinosa; Gloria del Solar; Miquel Coll


Book ID
117108905
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
210 KB
Volume
425
Category
Article
ISSN
0014-5793

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Structural features of the plasmid pMV15
✍ Paloma Acebo; Mario García de Lacoba; Germán Rivas; José M. Andreu; Manuel Espin 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 429 KB 👁 2 views

The small transcriptional repressor CopG protein (45 amino acids) encoded by the streptococcal plasmid pMV158 was purified to near homogeneity. Gel filtration chromatography and analytical ultracentrifugation showed that the native protein is a spherical dimer of identical subunits. Circular dichroi