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Overexpression, purification and properties of alcohol dehydrogenase IV from Saccharomyces cerevisiae

โœ Scribed by Christel Drewke; Michael Ciriacy

Book ID
119119669
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
491 KB
Volume
950
Category
Article
ISSN
0167-4781

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An NAD-dependent glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate:NAD+ oxidoreductase, EC 1.1.1.8) has been isolated and purified from Saccharomyces cerevisiae by affinity and exclusion chromatography. The enzyme was purified 5 1 OO-fold to a specific activity of 158. It has a molecular w