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Overexpression, Purification, and Crystallization of the Membrane-Bound Fumarate Reductase from Escherichia coli

✍ Scribed by César Luna-Chavez; Tina M. Iverson; Douglas C. Rees; Gary Cecchini

Book ID
115645656
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
152 KB
Volume
19
Category
Article
ISSN
1046-5928

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By a recombinant DNA approach we have prepared Escherichia coli cytoplasmic membranes that are highly enriched in the terminal electron transfer enzyme fumarate reductase. This enzyme is composed of four nonidentical subunits in equal molar ratio. A 69,000-dalton covalent flavin-containing subunit a