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Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase

✍ Scribed by Jason J Reddick; Cynthia Kinsland; Robb Nicewonger; Todd Christian; Diana M Downs; Malcolm E Winkler; Tadhg P Begley


Publisher
Elsevier Science
Year
1998
Tongue
French
Weight
511 KB
Volume
54
Category
Article
ISSN
0040-4020

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✦ Synopsis


The overexpression, purification and characterization of 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (HMP kinase) and 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase (HMP-P kinase) ate described. Surprisingly HMP-P kinase also shows HMP kinase activity. These enzymes are useful reagents for the preparation of intermediates on the thiamin biosynthetic pathway.


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ChemInform Abstract: Overexpression, Pur
✍ Jason J. Reddick; Cynthia Kinsland; Robb Nicewonger; Todd Christian; Diana M. Do πŸ“‚ Article πŸ“… 2010 πŸ› John Wiley and Sons βš– 27 KB πŸ‘ 2 views

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