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Overexpression, Purification, and Biochemical Characterization of the Extracellular Human CD83 Domain and Generation of Monoclonal Antibodies

✍ Scribed by Matthias Lechmann; Elisabeth Kremmer; Heinrich Sticht; Alexander Steinkasserer


Book ID
115645997
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
221 KB
Volume
24
Category
Article
ISSN
1046-5928

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## Abstract Seven monoclonal antibodies were raised against the immunoglobulin‐like extracellular domain of P0 (P0‐ED), the major protein of peripheral nervous system myelin. Mice were immunized with purified recombinant rat P0‐ED. After fusion, 7 clones (P01–P07) recognizing either recombinant, ra