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Overexpression of Trehalose Synthase and Accumulation of Intracellular Trehalose in 293H and 293FTetR:Hyg Cells

✍ Scribed by Guifang Lao; Deborah Polayes; Jiu Lin Xia; Fredric R. Bloom; Fred Levine; Jonathan Mansbridge

Book ID
115595743
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
98 KB
Volume
43
Category
Article
ISSN
0011-2240

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Determination of Intracellular Trehalose
Determination of Intracellular Trehalose and Glycogen in Saccharomyces cerevisiae
✍ U. Schulze; M.E. Larsen; J. Villadsen πŸ“‚ Article πŸ“… 1995 πŸ› Elsevier Science 🌐 English βš– 574 KB

A simple, sensitive and nonlaborious enzyme-based method has been developed for determination of both trehalose and glycogen in yeast cells. The method is based on extraction of trehalose and glycogen into a \(40 \mathrm{~mm}\) acetate buffer ( \(\mathbf{p H} 4.8\) ) by mechanical disintegration of