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Overexpression of Serratia marcescens lipase in Escherichia coli for efficient bioresolution of racemic ketoprofen

✍ Scribed by Zhang-De Long; Jian-He Xu; Li-Li Zhao; Jiang Pan; Sheng Yang; Ling Hua


Book ID
113804189
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
456 KB
Volume
47
Category
Article
ISSN
1381-1177

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Inactivation of the Serratia marcescens
✍ Nakamura, Kenzo ;Inouye, Masayori πŸ“‚ Article πŸ“… 1981 πŸ› Springer 🌐 English βš– 731 KB

A pBR322-derived plasmid pKEN221 carrying a Serratia marcescens lpp gene overproduces the outer membrane lipoprotein in an Escherichia coli lpp- cell. However, when this strain was continuously cultured in a rich medium for about thirty generations, many Lpp- mutants were accumulated. Out of six mut