Overexpression of ciliary neurotrophic factorin vivo rescues chick ciliary ganglion neurons from cell death
✍ Scribed by Finn, Tom P. ;Kim, Songte ;Nishi, Rae
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 297 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0022-3034
No coin nor oath required. For personal study only.
✦ Synopsis
Ciliary ganglion (CG) neurons untivity three-to fourfold in the embryonic eye rescued dergo target-dependent cell death during embryonic an average of 31% of the neurons that would have development. Although ciliary neurotrophic factor normally died in vivo. In some individuals, nearly all (CNTF) was identified in vitro by its ability to support of the neurons were rescued. ChCNTF had no effect the survival of chick CG neurons, its function as a on the number of neurons observed prior to cell death, target-derived neurotrophic factor has been quesnor were there any deleterious effects of either viral tioned by those working on mammalian-derived forms infection or overexpression of CNTF. These results of CNTF. We have purified and cloned a chicken show that chCNTF is able to function in vivo as a CNTF [chCNTF; formerly growth-promoting activity trophic factor for CG neurons, and suggest that lim-(GPA)] that is expressed in CG targets during the ited availability of trophic support is one of the factors period of cell death and is secreted by cells transfected regulating CG neuron survival during development. with chCNTF. In the present study we used a retrovi-