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Overexpression of 14-3-3γ causes polyploidization in H322 lung cancer cells

✍ Scribed by Wenqing Qi; Xiaobing Liu; Weiming Chen; Qiang Li; Jesse D. Martinez

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
437 KB
Volume
46
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

The 14‐3‐3 proteins are a family of highly conserved proteins that participate in a wide variety of cellular processes. Mounting evidence suggests that 14‐3‐3 proteins have a role in human cancers, however their role in tumorigenesis is unclear. Here we report that over‐expression of 14‐3‐3 γ protein in human lung cancer cell line H322 results in abnormal DNA replication and polyploidization. Cells that overexpress 14‐3‐3 γ are resistant to microtubule inhibitors and can reenter the cell cycle in the absence of mitosis suggesting that elevated levels of 14‐3‐3 γ may enable cells to bypass the mitotic checkpoint. Taken together, our data indicate that 14‐3‐3γ may contribute to tumorigenesis by promoting genomic instability. © 2007 Wiley‐Liss, Inc.

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