Overexpression and purification of full-length acidic fibroblast growth factor inE.coli

✍ Scribed by Sang-Uk Nham; Hyo-Soon Kim; Young-Ik Lee

• Book ID: 104636377
• Publisher: Springer Netherlands
• Year: 1994
• Tongue: English
• Weight: 391 KB
• Volume: 16
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00136467

✦ Synopsis

A full-length aFGF cDNA was cloned and inserted into a T7 expression vector for heterologous expression in E. coli. For high level production of aFGF, an optimal condition for overexpression (incubation at 42 OC for 2 hours) and a simple purification procedure employing in vitro refolding and an affinity chromatography was established. The production yield was 40 -50 ~g/mg of inclusion body preparation and purified aFGF showed mitogenic activity.