Over-expression of c-myc increases the sensitivity of epstein-barr virus immortalized lymphoblastoid cells to non-MHC-restricted cytotoxicity
✍ Scribed by Laura Cuomo; Quian-Jin Zhang; Sigurbjörg Torsteinsdottir; George Klein; Maria G. Masucci; Luigia Lombardi; Riccardo Dalla-Favera
- Book ID
- 102867889
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- French
- Weight
- 666 KB
- Volume
- 53
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Epstein-Barr virus (EBV)-carrying
Burkitt lymphoma (BL) lines which maintain the phenotypic characteristics of the in vivo tumor cells are more sensitive to natural (NK), interferonactivated (IAK) and IL-2-activated (LAK) cytotoxicity than EBV-immortalized lymphoblastoid cell lines (LCL) of normal 6-cell origin. All BL cells carry chromosomal translocations which lead to deregulated expression of the c-myc oncogene. LCLs transfected with constitutively active c-myc alleles display changes in growth properties and surface phenotype. In this study, we have examined the effect of c-myc deregulation on the sensitivity of L C L to NK, IAK and LAK effectors. C-myctransfected L C L showed an increased sensitivity to lysis which correlated with the level of c-myc expression. Expression of