Over a decade of experience with preimplantation genetic diagnosis
β Scribed by Yury Verlinsky; Jacques Cohen; Santiago Munne; Luca Gianaroli; Joe Leigh Simpson; Anna Pia Ferraretti; Anver Kuliev
- Book ID
- 118443648
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 35 KB
- Volume
- 82
- Category
- Article
- ISSN
- 1556-5653
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Preimplantation genetic diagnosis (PGD) was attempted in 12 couples in whom both parents carry the common AF508 deletion causing cystic fibrosis (CF). In vitro fertilization (IVF) was followed by cleavage stage biopsy on days 2 and 3 and removal of one or two cells for genetic analysis by nested pol
Three cases of preimplantation genetic diagnosis (PGD) (two for sexing and one for aneuploidy screening) are presented. Embryo biopsy was performed at day 3 and diagnosis was established with fluorescent in situ hybridization (FISH). Embryos not used for replacement were cultured in sequential media