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Ouabain-insensitive Na-ATPase activity in the basolateral membrane from rat jejunum

✍ Scribed by M.N. Orsenigo; M. Tosco; G. Esposito; A. Faelli


Book ID
108331960
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
538 KB
Volume
20
Category
Article
ISSN
0020-711X

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Ouabain-insensitive active sodium transp
✍ M. Tosco; M. N. Orsenigo; G. Esposito; A. Faelli πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 689 KB

The basolateral membrane of the jejunal enterocyte of the rat was separated by self-orienting Percoll-gradient centrifugation and further purified from brush border contamination. Pellets were analysed for Mg-, Na-and (Na, K)-ATPase activities. The uptake of 0.02 M NaCl was also followed by the rapi