Osteogenic protein-1 (OP-1, BMP-7) induces osteoblastic cell differentiation of the pluripotent mesenchymal cell line C2C12

✍ Scribed by Lee-Chuan C. Yeh; Alicia D. Tsai; John C. Lee

• Publisher: John Wiley and Sons
• Year: 2002
• Tongue: English
• Weight: 499 KB
• Volume: 87
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.10315

✦ Synopsis

Abstract

The effects of Osteogenic Protein‐1 (OP‐1, BMP‐7) on the differentiation of the pluripotent mesenchymal cell line, C2C12, were examined. OP‐1 at 50 ng/ml partially inhibited myotube formation in C2C12 cells, while OP‐1 at 200 ng/ml completely inhibited myotube formation and induced the formation of cells displaying osteoblastic morphology. High concentrations of OP‐1 elevated the alkaline phosphatase (AP) activity dramatically, both as a function of time and OP‐1 concentration. Osteocalcin (OC) mRNA expression was detected as early as 8 days in OP‐1‐treated cultures and subsequently increased considerably. Expression of bone sialoprotein (BSP) mRNA was low in control cultures and stimulated by OP‐1. Collagen type I mRNA expression was enhanced by OP‐1 during the early days in culture, but gradually decreased thereafter. MyoD mRNA expression, high in control cultures, was suppressed by OP‐1 in a dose‐ and time‐dependent manner. OP‐1 enhanced ActR‐I mRNA expression and significantly elevated the mRNA expressions of BMP‐1, BMP‐4, BMP‐5, GDF‐6, and GDF‐8. The present results indicate that OP‐1 is a potent inducer of C2C12 differentiation into osteoblastic cells. J. Cell. Biochem. 87: 292–304, 2002. © 2002 Wiley‐Liss, Inc.