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Osmotic water permeability in glycoprotein containing liposomes

✍ Scribed by Vassil Z. Neitchev; Aleksei P. Kostadinov

Publisher
Springer
Year
1987
Tongue
English
Weight
403 KB
Volume
12
Category
Article
ISSN
0301-4851

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✦ Synopsis

The kinetics of osmotic water permeability in proteoliposomes containing uracid glycoprotein was investigated by means of stopped-flow spectrophotometry. A biphasic time-course of scattered light with time was registered. The rate constants calculated from fits to an exponential function in the first phase were proportional to the final medium osmolarity. The apparent second order rate constants Kap p (Osm -j sec -1) were determined at different gIycoprotein concentrations in the original mixture for preparation of proteoliposomes. The value of Kap p at lipid:glycoprotein weight ratio = 1 was plotted in Arrhenius coordinates. The calculated activation energy for water permeation through the lipid bilayer suggests that eventual channel mechanism may be involved due to the presence of glycoprotein molecule in the liposomes.

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