โ Scribed by T. Hastings Wilson; Walter E. Landmesser Jr.
No coin nor oath required. For personal study only.
I NCREASED osmotic resistance of erythrocytes from patients with carcinoma was first noted by Chanel, about 1880, and confirmed by Janowsky and Lang in 1901and 1902. Kiinstler (1933), in reviewing the literature, discussed 350 cases of carcinoma reported by various workers and added seventytwo of his own. Although there was great variation in the methods employed by the several investigators, results, on the whole, showed that red cells from patients with carcinoma were more resistant to osmotic hemolysis than those from patients with benign lesions or those of normal subjects. Kunstler, however, concluded that the use of the hypotonic saline fragility test as a diagnostic tool for the early detection of cancer was not feasible. He believed that the resistance found was due to the marked anemia and cachexia present in almost all of the cases studied. We have found no subsequent reports in this field in the literature.
Inasmuch as the methods previously used for the study of osmotic resistance have been lacking in reproducibility, it has seemed worthwhile to reinvestigate the problem using the more reliable technique described by Jacobs, et al. This method utilizes buffered solutions to maintain a constant p H and a water bath for accurate temperature control.
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