Osmolarity Regulates Gene Expression in Intervertebral Disc Cells Determined by Gene Array and Real-Time Quantitative RT-PCR
✍ Scribed by Lawrence M. Boyd; William J. Richardson; Jun Chen; Virginia B. Kraus; Alok Tewari; Lori A. Setton
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 100 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0090-6964
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
Real-time RT-PCR method was exploited to identify endogenous reference genes in differentiating human T helper cells. When using this technology in our experimental system, finding a set of genes whose mRNA expression levels would not change appeared to be very challenging. Our initial plan to use t
## Abstract The goal of the investigation was to test the hypothesis that the phosphoinositide‐3 kinase (PI3K)/AKT signaling pathway regulates the expression of the major extracellular matrix component of the intervertebral disc, aggrecan, in nucleus pulposus cells. Primary rat nucleus pulposus cel
## Abstract __O__^6^‐methylguanine‐DNA methyltransferase (MGMT) mRNA expressions were examined in 100 neuroepithelial tumors by real‐time quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) using SYBR Green I. The mean relative quantitation value of MGMTmRNA normalized to the leve