Orthotopic implantation of human prostate cancer cell lines: A clinically relevant animal model for metastatic prostate cancer
✍ Scribed by Rembrink, Klaus; Romijn, Johannes C.; van der Kwast, Theo H.; Rübben, Herbert; Schröder, Fritz H.
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 756 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0270-4137
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✦ Synopsis
BACKGROUND.
To study the metastatic behavior of human prostate cancer cell lines, orthotopic injection in nude mice was performed. METHODS. Local tumor growth, metastasis formation, prostate-specific antigen, and androgen receptor expression were examined. RESULTS. Hormone-independent cell lines (PC-3, PC-3-125-1L, and TSU-Pr1) were highly tumorigenic and had a higher rate of lymph node metastasis after orthotopic than after subcutaneous implantation. PC-3 cell lines also consistently metastasized to the lungs. The androgen-sensitive LNCaP cell line showed local growth in 7 of 10, and lymph node metastasis in 4 animals. Significant serum PSA levels and strong receptor expression in primary and metastatic tumor tissues were observed. CONCLUSIONS. These results demonstrates that orthotopic implantation of human prostate cancer cell lines, including LNCaP, reproducibly leads to metastasis formation in nude mice.