Orotic acid quantification in dried blood spots and biological fluids by hydrophilic interaction liquid chromatography tandem mass spectrometry

✍ Scribed by Oceania D'Apolito; Daniela Garofalo; Giuseppe Paglia; Alfredo Zuppaldi; Gaetano Corso

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 277 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900758

✦ Synopsis

Abstract

Orotic acid (ORA) is an intermediate metabolite in the pathway of pyrimidine nucleotides; its urinary excretion is useful to diagnose the hereditary orotic aciduria and some hyperammonemic inherited defects of urea cycle enzymes and amino acid transporters. ORA analysis is based on stable isotope dilution by GC‐MS or LC‐MS/MS methods. We developed a fast assay that measures the ORA in dried blood spots (DBS), plasma and urine using hydrophilic interaction LC‐MS/MS. Within‐ and between‐day analytical imprecision (CV%) of three quality control levels, in plasma, DBS and urine, ranged from 0.8 to 14.1%, while the inaccuracy ranged from −13.5 to 9.4%. In healthy children (n=20), ORA concentrations were less than 0.69 μM in plasma, less than 0.82 μM in DBS and from 0.2 to 1.4 mmol/mol of creatinine in urine. A patient with citrullinemia showed ORA levels of 133 μM in plasma and 39 μM in DBS. A patient with hyperammonemia‐hyperornithinemia‐homocitrullinemia (HHH) syndrome presented a urinary ORA level of 9.1 mmol/mol of creatinine. The method is potentially able to discriminate affected patients from reference subjects; the clinical validation should be expanded on a higher number of patients.